【文獻(xiàn)标題(tí)】Selenium Mitigates Ammonia Induced Neurotoxicity by Suppressing Apoptosis, Immune Imbalance, and Gut Microbiota Driven Metabolic Disturbance in Fattening Pigs
【作者】Yutao Li1, Jing Wang et.al
【作者單(dān)位】東(dōng)北師範大學( Northeast Normal University)
The hypothalamus tissues were mixed with cold PBS at the ratio of 1:9 (w:v) and homogenized to obtain the fresh supernatant, which was centrifuged at 4500 r·min?1 for 18?min, at 4 ℃. The contents of IL-6, IL-10, tumor necrosis factor (TNF)-α, IFN-γ, NO, and iNOS were detected using the specifc enzyme-linked immunosorbent assay kits (Hengyuan, China).