恒遠産品文獻:雞GP,GS,GK等指标ELISA試劑盒引用文獻

【文獻(xiàn)标題(tí)】Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

【作者】Longlong Li ,Mengling Peng ,Chongyang Ge, et.al

【作者單(dān)位】南京農(nóng)業大學（Nanjing Agricultural University）

【文獻中引用産品】

雞(jī)糖原磷酸化酶(GP)ELISA試劑(jì)盒

雞(jī)糖原合成酶(GS)ELISA試劑(jì)盒

雞(jī)葡萄糖激酶(GK)ELISA試劑(jì)盒

雞(jī)磷酸果糖激酶-1（PFK-1）ELISA試劑(jì)盒

雞(jī)丙酮酸激酶（PK）ELISA試劑(jì)盒

雞(jī)丙酮酸脫氫酶E1(PDH E1)ELISA試劑(jì)盒

雞(jī)檸檬酸合成酶(CS)ELISA試劑(jì)盒

雞(jī)烏頭酸酶(ACO)ELISA試劑(jì)盒

雞(jī)磷酸烯醇式丙酮酸羧激酶（PEPCK）ELISA試劑(jì)盒

雞(jī)ATP-檸檬酸裂解酶(ACLY)ELISA試劑(jì)盒

雞(jī)NADH脫氫酶ELISA試劑(jì)盒

雞(jī)ATP合酶（ATP）ELISA試劑(jì)盒

【關(guān)鍵詞(cí)】Hydroxycitric acid  Acetyl-CoA  ATP-citrate ? Energy metabolism Primary chicken hepatocytes

【DOI】 10.1159/000481564

【影響(xiǎng)因子(IF)】11.7

【出版期刊】《CELLULAR PHYSIOLOGY AND BIOCHEMISTRY》

【産品原文引用】

Measurement of the key glucose metabolic enzyme protein content

Cells were cultured in 6-well plates (2×106 cells per well) and treated with 0, 1, 10 or 50 μM (-)-HCA for 24 h. The cells were harvested and disrupted ultrasonically in ice and then centrifuged at 3000 rpm for 20 min at 4 °C. The supernatants were collected, the protein concentrations of glycogen phosphorylase (GP) (catalog #: E-75175), glycogen synthase (GS) (catalog #: E-75174), glucokinase (GK) (catalog #: E-76111), phosphofructokinase-1 (PFK-1) (catalog #: E-76131), pyruvate kinase (PK) (catalog #: E-76565), pyruvate dehydrogenase (PDH, E1) (catalog #: E-76118), citrate synthase (CS) (catalog #: E-75165), aconitase (ACO) (catalog #: E-75144) and phosphoenolpyruvate carboxykinase (PEPCK) (catalog #: E-75117) were measured using ELISA kits according to the manufactures’ protocols (Shanghai Hengyuan Biological Technology Co., China). The enzyme protein content was normalized to total protein concentration in the sample.

完整版PDF文獻請(qǐng)咨詢在線客服或者聯(lián)系我司業務員！

更多公司福利請(qǐng)關(guān)注“恒遠生物"微信公衆号！