2.3. In vitro modeling of alcohol-induced ALD and EGCG intervention
Metabolism evaluation. After culturing for 7, 14 and 21 days, the cell culture supernatants of ALD, EGCG and control samples were taken for
ELISA detection, and the metabolic status of albumin (ALB), carbamide (CARB) and glutathione (GSH) was quantitatively analyzed by the test kit (Shanghai Hengyuan Biotechnology Co., Ltd) according to the instructions.