恒遠産品文獻:蚯蚓CYP450,CarE,GST,ELISA試劑盒引用文獻

【文獻(xiàn)标題(tí)】Enantioselective bioaccumulation and detoxification mechanisms of earthworms (Eisenia fetida) exposed to mandipropamid

【作者】Kuan Fang , Lingxi Han , Yalei Liu ,et.al

【作者單(dān)位】中國(guó)農業科學院（Chinese Academy of Agricultural Sciences）

【文獻中引用産品】

蚯蚓細胞色素P450(CYP450)ELISA試(shì)劑(jì)盒 酶活

蚯蚓羧酸酯酶(CarE)ELISA試(shì)劑(jì)盒 酶活

蚯蚓（GST）ELISA試(shì)劑(jì)盒 酶活

【關(guān)鍵詞(cí)】Enantioselectivity,Mandipropamid enantiomers,Detoxification enzymes,Transcriptome sequencing,Detoxification mechanisms

【DOI】doi.org/10.1016/j.scitotenv.2021.149051

【影響(xiǎng)因子(IF)】10.5

【出版期刊】《Science of the Total Environment》

【産品原文引用】

2.4. Determination of detoxification enzyme activities and the secondgeneration integrated biomarker response index (IBRv2)

One earthworm was randomly selected from each beaker, weighed,and homogenized with a 50 mM phosphate buffer (pH 7.8; 1:10, W/V). The homogenized mixture was then centrifuged in a cryogenic centrifuge at 9000 rpm for 10 min, and the collected supernatant was analyzed to determine the activities of CYP450, CarE, and GST using a kit provided by ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The absorbance (optical density value) was determined at a wavelength of 450 nm using a microplate reader, and the activities of CYP450, CarE, and GST in the sample were calculated from the standard curve.

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