技術文獻:豬IL-4, IL-6,IgG,IgM引用文獻

【文獻(xiàn)标題(tí)】Excessive ammonia inhalation causes liver damage and dysfunction by altering gene networks associated with oxidative stress and immune function

【作者】Xiangyin Zeng , Runze Liu , Yutao Li , et.al

【作者單(dān)位】東(dōng)北農業大學（Northeast Agricultural University）

【文獻中引用産品】

豬白介素4(IL-4)ELISA試(shì)劑(jì)盒

豬白介素6(IL-6)ELISA試(shì)劑(jì)盒

豬白介素8(IL-8)ELISA試(shì)劑(jì)盒

豬免疫球蛋白G(IgG)ELISA試(shì)劑(jì)盒

豬免疫球蛋白M(IgM)ELISA試(shì)劑(jì)盒

【關(guān)鍵詞(cí)】Ammonia ,Liver damage ,Oxidative stress ,Immune dysfunction ,Gene network

【DOI】doi.org/10.1016/j.ecoenv.2021.112203

【影響(xiǎng)因子(IF)】6.2

【出版期刊】《Ecotoxicology and Environmental Safety》

【産品原文引用】

2.5. Measurement of immune function 

To assess the effect of ammonia exposure on immune function, serum samples were used to analyze cytokine and immunoglobulin levels at Northeast Agricultural University. The cytokines (IL-4, IL-6 and IL-8) were determined by ELISA kits according to the kit instructions (Shanghai Hengyuan Biotechnology Co., Ltd, China). All cytokines were reported in ng/L. Inter and intra assay cytokine CVs were IL-4 (14.67%;< 16%), IL-6 (10.12%; < 13%) and IL-8 (13.1%;< 16%). Serum immunoglobulin-A (IgA), IgG and IgM were detected by ELISA kits using the enzyme-labeled instrument (SpectraMax? ABS00254, California, USA). All immunoglobulin levels were expressed in mg/mL. IgA ELISA kit was purchased from ShangHai Yanjin Biotechnology Co., Ltd (China). IgG and IgM ELISA kits were detected by ELISA kits (Shanghai Hengyuan Biotechnology Co., Ltd, China). 

完整版PDF文獻請(qǐng)咨詢在線客服或者聯(lián)系我司業務員！

更多公司福利請(qǐng)關(guān)注“恒遠生物"微信公衆号！