技術資訊：人TNF-α,IL-1β,E2 ELISA試劑盒引用文獻

【文獻(xiàn)标題(tí)】Dehydroepiandrosterone alleviates intestinal inflammatory damage via GPR30-mediated Nrf2 activation and NLRP3 inflammasome inhibition in colitis mice

【作者】Ji Cao , Miaomiao Lu , Weiyuan Yan , et.al

【作者單(dān)位】南京農(nóng)業大學（Nanjing Agricultural University）

【文獻中引用産品】

人腫瘤壞(huài)死因子α(TNF-α)ELISA試劑(jì)盒

人白細胞介素1β(IL-1β)ELISA試(shì)劑(jì)盒

人雌二醇(E2)ELISA試(shì)劑(jì)盒

【關(guān)鍵詞(cí)】Dehydroepiandrosterone ,GPR30 ,Nrf2 ,NLRP3 inflammasome ,Colitis

【影響(xiǎng)因子(IF)】7.37

【出版期刊】《Free Radical Biology and Medicine》

【産品原文引用】

Sigma (St Louis, MO, USA) provided the DHEA, dimethyl sulfoxide (DMSO) and LPS (Escherichia coli 055:B5). The Opti-MEM medium and fetal bovine serum (FBS) were obtained from Gibco (Erie, NY, USA). The dulbecco's modified Eagle's medium (DMEM) and trypsin-EDTA were all obtained from Biological Industries (Kibbutz Beit-Haemek, Israel). Corning (NY, USA) provided the cell culture dishes.

TRIZOL Reagent Kits, SYBR Green Master Mix and reverse transcriptase kit were all purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). Jiancheng Bioengineering Institute of Nanjing (Nanjing, China) provided the Nitric Oxide (NO), malondialdehyde (MDA) and total superoxide dismutase (SOD) detection kits. Shanghai hengyuan biological technology Co., Ltd. (Shanghai, China) provided the TNF-α, IL-1β and Estradiol (E2) ELISA kits. Beyotime Biotechnology Institute (Shanghai, China) provided the glutathione peroxidase, nitric Oxide (NO), protein contents assay kits and N-Acetyl-L-cysteine (NAC). MCE (St. Louis, MO, USA) provided the inhibitors including G1, G15, SB203580, U0126, BAY11-7082, GKT137831, TAK-242, 3-MA, Fulvestrant, Flutamide, ML385 and RTA-408.

 







  

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